Yokogawa Electric Corporation's Latest Single Cell Analysis Solution ™ The system SS2000 has been used by researchers from the University of Surrey in the UK for groundbreaking research in the emerging field of single-cell lipidomics, and related articles have been published in the renowned academic journal Analytical Chemistry. SS2000 is a live cell imaging device equipped with Yokogawa Electric's unique dual microlens turntable imaging technology, capable of conducting cutting-edge life science research.
*Single cell non targeted lipidomics using liquid chromatography and visual data collection after selecting live cells | Analytical Chemistry: https://pubs.acs.org/doi/10.1021/acs.analchem.3c05677
Figure 1: Single Cell System SS2000
Lipidomics is a large-scale study of lipids. Lipids are a large class of molecules that play a biological role from maintaining normal human operation to the development of major diseases. Typically, lipidomics research is conducted by extensively isolating and analyzing lipids from many cells. Although extensive lipid analysis can provide scientists with a population average of lipid mass spectrometry, this method cannot distinguish subtle differences between cells, nor can it reveal spatial or temporal differences in lipid profiles caused by intercellular interactions.
Single cell lipidomics is an emerging field aimed at analyzing the lipid composition of single cells. In addition to intercellular differences, these studies also provide scientists with methods to explore spatial and temporal differences, helping to overcome the challenges faced by large-scale lipidomics research. Understanding these differences is key to creating more complete images and understanding diseases such as cancer. One of the challenges faced by researchers is to isolate individual cells while maintaining their natural lipid profile. The current single-cell separation method involves simultaneously isolating and suspending multiple cells, and then separating them through narrow channels. However, this may cause significant pressure on the cells and may lead to changes in their lipid composition. Yokogawa Electric's Single Cell System SS2000 utilizes confocal imaging technology to help eliminate challenges in single-cell lipidomics. It is a dual-purpose system that can perform live cell imaging, as well as fully automatic single cell and subcellular sampling without undergoing suspension processes, thereby minimizing pressure on cells as much as possible.
SS2000 Application Example
Figure 2: Before sampling Figure 3: After sampling
Yokogawa Electric's collaborators at the University of Surrey have demonstrated that Yokogawa Electric's SS2000 has made significant improvements in single-cell lipidomics compared to other single-cell separation technologies. SS2000 can sample adherent cells in culture, and researchers can maintain the natural lipid mass spectrometry of the sampled cells, while also providing spatial and temporal information based on real-time imaging. Researchers have utilized this unique dual capability to create workflows that other technologies cannot achieve. They will combine the spatial and temporal information obtained from the SS2000 dual microlens turntable imager with liquid chromatography mass spectrometry data for each sampled cell. This new method is a fundamental step forward in the field of lipomics, and will help to make breakthroughs in alternative treatment and therapy of cancer, diabetes and cardiovascular diseases * 2.
Through this approach, Yokogawa Electric will continue to provide customers with new value and the latest technology, contributing to ensuring the well-being of everyone.
*Wang Zehua, Cao Mingjun, Sin Man Lam, Shui Guanghou, Embrace Lipomics with Single Cell Resolution: Prospects and Hidden Dangers [J] Tracking Trends in Analytical Chemistry, Volume 160, 2023116973, ISSN 0165-9936, https://doi.org/10.1016/j.trac.2023.116973
More information
Scientists at the University of Surrey study lipids cell by cell, making new cancer research possible | University of Surrey: https://www.surrey.ac.uk/news/new-cancer-research-made-possible-surrey-scientists-study-lipids-cell-cell
Single Cell ™ System SS2000: https://www.yokogawa.com/cn/solutions/products-and-services/life-science/single-cellome/ss2000/
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